Nana Mizuki - Rue/Princess Kraehe
Nanae Katou - Ahiru/Princess Tutu
Naoki Yanagi - Mythos
Takahiro Sakurai - Fakir
Akiko Hiramatsu - Edel
Erino Hazuki - Uzura
Kyoko Kishida - Narration
Noboru Mitani - Drosselmeyer
Sachi Matsumoto - Pike
Takayuki Sugo - The Raven
Yasunori Matsumoto - Neko-sensei
Yuri Shiratori - Lilie
Yuu Urata - Autor
Akemi Kanda - Arikuimi
Akira Tomisaka - Yanko
Asuka Tanii - Tsuchibutannu
Atsushi Kisaichi - Pualo
Eisuke Asakura - Lysander
Hiroaki Shimoura - Chihuajuan
Hiromi Ohtsuda - Fakir
Kaori Matoi - Tsuchibutaana
Mugihito - Karon
Papaya Suzuki - Femio
Rie Ishizuka - Ebine